RT PCR System

Built on over 10 years of real-time expertise, the Applied Biosystems 7300 Real-Time PCR System is an affordable platform for the detection and quantification of nucleic acid sequences that will not compromise your data quality or dye choice flexibility. The 7300 Real-Time PCR System combines thermal cycling, fluorescence detection, and application-specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube, homogeneous reaction. Quantitative results are available immediately upon completion of PCR, with no need to run gels, purify PCR products, or perform any post-PCR manipulations. Real-time PCR runs are completed in under two hours, using 96-well plates and tubes (individual or 8-strip), with a supported volume range of 20 – 100 ?L. Compared with manual PCR quantitation techniques such as Northern blotting or RNase protection assays, real-time PCR offers enormous time-savings, greater sensitivity, superior precision, and a larger dynamic range.