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UCL Proteomics Facility

Institute for Women's Health

CONTACT 1 John Timms
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Description

The Cancer Proteomics Laboratory led by Dr John Timms was established 5 years ago. The laboratory's main research focus is on cell signalling in cancer and the identification of cancer biomarkers through the application of quantitative proteomic methodologies and biochemical and molecular biology techniques. Facilities include a biochemistry laboratory, dedicated clean rooms for high-sensitivity, low-contamination sample handling, fluorescence 2D difference gel electrophoresis (2D-DIGE) capability, liquid-handling robotics and liquid chromatography (LC) for protein and peptide separations. Mass spectrometry (MS) instrumentation for expression profiling, protein/peptide identification and characterisation includes a Thermo LTQ Orbitrap XL hybrid mass spectrometer with on-line Dionex3000 nano-LC system and Bruker Ultraflex MALDI-TOF/TOF instrument.

Associated Items

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    Agilent HPLC

    Agilent 1100 series HPLC with fraction collector used for off-line protein and peptide separations.
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    • Manufacturer: Agilent
    • Technique: liquid chromatogrpahy
    • Organisational Unit: Institute for Women's Health
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    Bio-Rad Imager

    Biorad Molecular Imager FX incorporating phosphorimager
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    • Manufacturer: Bio-Rad
    • Technique: phosphorimaging
    • Organisational Unit: Institute for Women's Health
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    MALDI-TOF MS

    Bruker Ultraflex MALDI-TOF for high-throughput MALDI-based mass spectrometry
    more details »

    • Manufacturer: Bruker
    • Technique: MALDI-TOF MS
    • Organisational Unit: Institute for Women's Health
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    Mulitwavelength Imager

    Typhoon 9400 multiwavelength Imager for small to large format fluorescence imaging.
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    • Manufacturer: Amersham Biosciences
    • Technique: Fluorescence imaging, 2D-DIGE
    • Organisational Unit: Institute for Women's Health
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    nLC system

    Dionex Ultimate 3000 nano LC system used for on-line (to MS) peptide separations by reversed-phase LC (C18) . Linked to an LTQ Orbitrap XL mass spectrometer.
    more details »

    • Manufacturer: Dionex
    • Technique: liquid chromatogrpahy
    • Organisational Unit: Institute for Women's Health
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    Tecan liquid handling robot

    Tecan Aquarius 96 tip liquid-handling robot and tip loader
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    • Manufacturer: Tecan Genesis
    • Technique: liquid-handling
    • Organisational Unit: Institute for Women's Health

Item ID #2531.

Last Updated: 20th January, 2014

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